Estos son los 29 equipos de la MLS:
Conferencia Este:
1. Atlanta United FC
2. Chicago Fire FC
3. FC Cincinnati
4. Columbus Crew SC
5. DC United
6. Inter Miami CF
7. CF Montréal
8. Nashville SC
9. New England Revolution
10. New York City FC
11. New York Red Bulls
12. Orlando City SC
13. Philadelphia Union
14. Toronto FC
15. Charlotte FC
Conferencia Oeste
1. Austin FC
2. Colorado Rapids
3. FC Dallas
4. Houston Dynamo FC
5. Los Angeles Galaxy
6. Los Angeles FC
7. Minnesota United FC
8. Portland Timbers
9. Real Salt Lake
10. San Jose Earthquakes
11. Seattle Sounders FC
12. Sporting Kansas City
13. Vancouver Whitecaps FC
14. St. Louis City SC